Angelo Buono, the Hillside Strangler

Angelo Buono, the Hillside Strangler Angelo Anthony Buono, Jr. was one of the two Hillside Stranglers responsible for the 1977 kidnap, rape, torture and murder of nine girls and young women in the hills of Los Angeles, California. His cousin, Kenneth Bianchi, was his crime partner who later testified against Buono in an effort to avoid the death penalty. The Early Years Angelo Buono, Jr. was born in Rochester, New York, on October 5, 1934. After his parents divorced in 1939, Angelo moved to Glendale, California with his mother and sister. At a very early age, Buono began showing a deep disdain for women. He verbally assaulted his mother, a behavior that later intensified towards all women he encountered. Buono was brought up as Catholic, but he showed no interest in attending church. He was also a poor student and would often skip school, knowing that his mother, who had a full-time job, could do little to control his activities. By the age of 14, Buono had been in a reformatory and was bragging about raping and sodomizing young local girls. The Italian Stallion Beginning in his late teens, Buono married and fathered several children. His wives, who were at first attracted to his macho self-proclaimed Italian Stallion style, would quickly discover that he had a deep loathing for women. He had a strong sexual drive and would physically and sexually abuse  the women in his life. Inflicting pain seemed to add to his sexual pleasure and there were times that he was so abusive, many of the women feared for their lives. Buono had a small, semi-successful car upholstery shop attached to the front of his home. This offered him seclusion, which was what he needed to act out his sexual perversions with many of the young girls in the neighborhood. It was also where his cousin, Kenneth Bianchi, came to live in 1976. A Career Jump Into Pimping Buono and Bianchi embarked on a new career as small-time pimps. Bianchi, who was more attractive than his wiry, large-nosed cousin, would lure young runaway girls to the home, then force them into prostitution, keeping them captive with threats of physical punishment. This worked until their two best girls escaped. Needing to build up their pimp business, Buono purchased a list of prostitutes from a local prostitute. When he figured out he had been scammed, Buono and Bianchi set out for revenge, but could only find the prostitutes friend, Yolanda Washington. The pair raped, tortured and murdered Washington on October 16, 1977. According to authorities, this was Buono and Bianchis first known murder. The Hillside Strangler and Bellingrath Link Over the next two months, Bianchi and Buono raped, tortured and killed another nine women ranging in ages from 12 to 28. The press named the unknown killer as the Hillside Strangler, but police were quick to suspect that more than one person was involved. After two years of hanging around his piggish cousin, Bianchi decided to return to Washington and reunite with his old girlfriend. But murder was on his mind and in January 1979, he raped and murdered Karen Mandic and Diane Wilder in Bellingrath, Washington. Almost immediately the police linked the murders to Bianchi and they brought him in for questioning.  The similarities of his crimes to those of the Hillside Strangler was enough for the detectives to join forces with the Los Angeles detectives and together they question Bianchi. Enough evidence was found in Bianchis home to charge him with the Bellingrath murders. Prosecutors decided to offer Bianchi a life sentence, instead of seeking the death penalty, if he gave full details of his crimes and the name of his partner. Bianchi agreed and Angelo Buono was arrested and charged with nine murders. The End for Buono In 1982, after two lengthy trials, Angelo Buono was found guilty of nine of ten Hillside murders and received a life sentence. Four years into serving his sentence, he married Christine Kizuka, a supervisor at the California State Department of Employee Development and a mother of three. In September 2002, Buono died of a suspected heart attack while in Calipatria State Prison. He was 67 years old. Interesting Note: In 2007, Buonos grandson, Christopher Buono, shot his grandmother, Mary Castillo, then killed himself. Castillo was married to Angelo Buono at one time and the two had five children. One of the five children was Chris father.

Ideas And Themes In Writings English Literature Essay Essay Example

Ideas And Themes In Writings English Literature Essay Essay Example Ideas And Themes In Writings English Literature Essay Essay Ideas And Themes In Writings English Literature Essay Essay Writers use tonss of literary techniques and characteristics to back up their thoughts and subjects in their writings.All those tehniques are design to pull strings and determine the readers perceptions.They place readers to react to the authorship in certain ways, either holding with or challenging those themes.Themes of allienation, nostalgia to love and loss are really frequently repeated in Literature.We can see this really clear in three short narratives which I would wish to compose about, Erneast Hemingways Hills Like White Elephants , John Cheevers The Enormous Radio and Doriss Lessing To Room Nineteen.In first narrative Hemingway tells us about inabillity to pass on efficaciously in a relationhip.Main characters got a immense trouble in jointing their feelings which makes them frustrated with each other.In The Enormous Radio we see subjects such as dependence and loss of control, hidden secrets ans dirts and communicating issues but they all lead to the chief subject of t his narrative which is loss of love.Last Doris Lessing narrative subjects are subject of allienation, individuality issue and loss of sense of being.Main character of this narrative, Susan is portrayed as a captive of political orientation driven by sociaty that adult females are expected to be lief and volitionally acepting their repression and bondage. Narrative point of viewe is one of the first techniques which has immense influence on significance and tone of the story.All narratives are written in the same a 3rd individual point of viewe but with a small difference between them.Erneast Hemingway wrote Hills Like White Elephants in 3rd individual point of position and aim which is limited to what characters say and do.He does nt uncover their ideas ang feelings, as a readers we need to think that by construing the text.In Enormous Radio we see that storyteller use the same 3rd individual point of viewe but it s omniscient.He is able to depict non merely each characteraa‚Â ¬a„?s actions but besides their inside emotions, motivations, and feelings.Sometimes narrator even takes subjective attack by depicting one of the characters Irene as a egoistic adult females which is merely concerned about how people viewe her and by depicting Jim as a loyal and difficult working adult male who tries really difficult to give her and their kids a comfy life.In Doriss Lessings To Room Nineteen narrative starts from the 3rd individual narrative point of viewe merely to switch subsequently in a narrative to first individual point of viewe.Lessing utilizations watercourse of concioussness techinque and interior soliloquy which creates the feeling for the reader that he is about listen ining on the flow of concious experience in characters mind.This give a reader an acces to ideas, emotions and esthesiss experienced by a character. Varied inside informations of puting and character has got a immense contributon to the maning of these short stories.Setting plays the biggest function in Hemingways narrative where even the rubric refers to symbolical importance of it.Jig, one of the characters of a narrative, by depicting the hills that they look like white elephants refers to her gestation and the chief issue of this narrative which is abortion.She describes the hills in the distance that they look like white elephants because American which she travells with, views the babe as an aproaching obstruction to their careless life.The remainder of a scene besides provides symbolism which express the tenseness and struggle between the couple.The train paths traveling into two different waies, in between which twosome sits at the train station might mention to match being in the center of doing drastic determination. A twosome is merely like those paths which run side by side but will neer hold a opportunity to run into . Different sort of land on both sides, on one viridity and fertile and on the other dry and without trees besides describes their different viewe and reading of the quandary of pregnancy.In The Enormous Radio , the reaching of the new wireless which changes its scene, traveling deeper in the house, traveling from the door to the center of the life room behind the couch because of its physical ugnliness it seems to be like agressive interloper, irrupting Irenes life.By listening to the wireless which picks up noises and conversations from the whole edifice she besides starts irrupting on other people lifes.This is how we start to see immense alteration in Irene and her relationship with Jim.She gets involved in people lives heard on the wireless so much she shortly became down and alterations from a pleasant, instead apparent adult female to a adult female who doubts who she is and uncertainties in her relationship with her husband.They both start to gain there is a tenseness in th eir matrimony, that Irene has many dark, deep secrets which she has hidden really good all these old ages and which she feels guilty about.Setting it s non so of import in this story.Narrator is seeking to concentrate merely on the wireless, voices and music coming out of it which plays immense function in a narrative by determining ambiance of a narrative and besides altering temper of a chief characters.The same we will see in Doris Lessings narrative where there is less description on puting but this minimal art have immense importance for the meaning.This minimal art gives the reader a sense of unrealized emptiness of a relationshp between Susan and Matthew and emptiness in Susan s life which is described as a desert which shows her hopelessness and depressesion.Also the empty room 19 which in she spends most of a clip describes Susan s demand of allienation. Use of techniques such as sarcasm, symbolism an understatement by wrtiers in those short narratives has got a immense impact on themes.The most of import in those narratives is symbolism which is a usage of symbols to stand for or propose other things or ideas.As said before in Hills Like White Elephants we can already see symbolism in the rubric of a story.Jig refers to the hills that they look like white elephants to speak about abortion which is the chief subject of this story.A white elephant is a mostly useless object that is expenssive to ain and maitain which symbolise a unborn baby.The whole symbolism of a scene is of import in many ways which I already had a opportunity to analyze earlier.The rail paths could symbolize Jigs and Americans relationship and being in the center of doing an of import decision.Two sides of a station described in a narrative has it s ain significance, green can by a symbol of life, babe, a new beggining and dry side can stand for decease and abort ion.Irony in this narrative chiefly shows Jig s defeat towards the American and this is its chief purpose.In conversation between them about licuorice and its familiar gustatory sensation we can clearly see Jig is non merely mentioning to a drink but besides to their life style which she is able to abandon at any point to settle down.I believe immense impact on the narrative has the last sentence spoken by Jig, when she tells the American she is fine.We can understand this sentence as an sarcasm and that Jig will travel through with the abortion because of her inability to pass on her feelings to the American wich leaves her defeated and non being able to contend anymore.Also conversation between them, approximately such an of import topic which is abortion, seems to be really simple, fiddling and even pointless.The talk about good drinks and lovely hills is merely a screen for a deathly argument.This tehnique used by Hemingway is called understatement.Again symbolism is used in Joh ns Cheever The Enormous Radio.It is chiefly used to depict the concealed significance of the wireless from the title.It symbolise an dependence and loss of control.Irene gets so obssesed with listening to her friends and neighbors on the wireless that she is non even interested any longer in her relationships with other people in existent life.She prefers to travel place and eavsdropp on them through the radio.Radio besides symbolise a concealed secret and scandal.When Irene start to doubts felicity of her marriege, all secrets are being revealed like larceny and abortion, which she was concealing all these years.Radio is besides used as an irony.It was purchased to convey joy and felicity to Irenes and Jims life but it merely caused problem between them uncovering deep and dark secrets.In To the room 19 once more we can happen symbolism in the rubric of a story.We see Susan as a adult female who has been caught up in the web of the societal functions in a community and she is seek ing to step out of this universe into a universe of her ain devising which is room nineteen.She is no longer a married woman and female parent in this room, she escapes her societal function and she eventually finds peace and freedom.We can see an sarcasm in words depicting Susan and Matthew as intelligent which is the really characteristic that is their downfall.Although they do everything sanely in their heads, their repression of her as a adult female is anything but intelligent.Also Lessing usage repeat technique, utilizing word intelligence fiftee times to do an sarcasm even stronger. Dialogues, conversations between characters have besides large impact on the significance of those stories.Hemingway narrative is told in 3rd individual point of viewe and it does nt state us any facts about characters.This is why duologue is of import and we need to interpretate it to happen out about attitudes oh the chief characters towards each other.Their are able in this manner to talk for themselve and we can besides see through the tone and form of the duologue, exsistence of a deep job in their relationship.In The Enormous Radio duologue has the same purpose.We are able through duologue to happen out more about characters ideas and feelings.We besides in a duologue see a tenseness between chief characters, which bilds up towards the terminal of the story.In the To Room Nineteen Doris Lessing we can see through duologues between Susan and Matthew the deficiency of communicating in their married life.Lessing besides use interior soliloquy to emphasize the significance of the story.Through Susan s interior soliloquy we can see her helplensness and emptiness of her matrimony life. All those strories got their ain particular construction which has an impact on the temper and ambiance of these stories.In Hemingways Hills Like White Elephants puting frames the narrative, it plays a important function in finding the ambiance in his work. The pick of a scene and objects supports the sytuation and develops the narrative conflict.Also duologues are keeping the narrative together, they tell us about the chief subject of a story.In The Enormous Radio music frames the narrative, creates the temper and reveals the emotional passage of the characters.In To Room Nineteen Doris Lessing is utilizing more literary techniques in her authorship to back up the significance. Narrator by altering the tone of linguistic communication creates a mood.At the beggining of a authorship words are longer which makes reading slower and creates really peacfull effect.But than towards the terminal of a narrative storyteller is seeking to make tenseness in a narrative and starts utilizing shorter words which makes our reading much quicker.Also watercourse of conciousness technique adds dramatic consequence to the narrative and without it composing would be really bland. I have truly enjoyed reading short narratives, particularly those three which I have chosen for this rating and analysis.Mainly I believe so because of female issues in sociaty presented in those stories.Earnest Hemingways Hills Like White Elephants is deffinitelly my favourite.It is really interesting that storyteller does nt uncover any informations about characters, what they think and feel.To happen out we need to interpretate the text, in this instance duologue between them.Through reading the narrative I felt like sitting following to the twosome, eavsdropping on their conversation.Because there is a immense communicating issue between them and they are unable to uncover their true feelings once more it is left to the reader to analyze what characters are truly thinking.It besides intrigued me how narrative shows a contrast between male and female viewe on relationsips.When abortion for Jig, killing their unborn babe is a immense determination for her, for American is merely a simple operation letting the air in.He does nt place himself with the babe which is an obstruction for him and his careless lifestale. I besides found The Enormous Radio really interesting to read.I believe John Cheever through this narrative once more proved the fact how pass oning issues between people might impact their relationship.Irenes secrets and dirts creates tenseness between their matrimony and they lead to loss of trust and love between them.The construction of a narrative has besides catched my attention.I found it really interesting that music was making the temper in the narrative and that music was uncovering the emotional passage of the characters.In To Room Nineteen I was hugle intrested in Lessings attitude towards female repression, disaffection and flight which is all exposed in this story.She tries to explicate how societal, cultural, and ethic recreations, how they all limit adult females positions and experiences and their rational, religious, and emotiona l freedom.By indroducing us to the chief character Susan, which full exsistence was like prison, she tells us that this allienation might even ensue in mental breakdown.Short narratives are really speedy and gratifying reading but still they have a immense deepness and of import message hidden in them.I would deffinitely recomend to others short narratives which I had a opportunity to read and I will besides go on to spread out my experience as a reader.

The experiences of American Civil War Veterans in April 1865 Research Paper

The experiences of American Civil War Veterans in April 1865 - Research Paper Example Coming home after four years of war must have had a lasting impact on the veterans. Posttraumatic stress, loneliness and physical injury were some of the things they went through. In other words, they literally went through hell. It must have killed them inside to know that they had to put on a bright face to reassure their loved ones and the nation as well that everything was okay. As evidenced from the wide range of memoirs, autobiographies and civil war diaries present in the annals of American history, individual experiences of the soldiers varied. As their experiences varied, so did their sentiments, hopes and future aspiration. Perhaps, one thing that they shared was a sense of pride and nationalism. The war was finally over. Confederate General Robert Lee had surrendered his authority to the Union under LTG U.S. Grant on April 9, 1865. Background In order to achieve clarity, it is important to place the civil war into its proper historical context. This begins with an understa nding that the issue of slavery in the United States was in the center of the worst internal conflict the country has ever experienced. The plantation elite, most of who were in the South ripping the benefits of slavery and forced labor in their tobacco, wheat and sugarcane plantation lacked the enthusiasm to relinquish their slave. They openly opposed abolition and maintained that it was within their rights to own property. Their argument was that abolition was repugnant to the constitutional right to own property. However, due to the rise of humanitarianism, the issue of slave ownership came under sharp criticism amid calls for the abolition of slavery. The anti-slavery campaign was the ethical cornerstone of the Republican presidential campaign in 1860 spearheaded by Abraham Lincoln1. Upon ascension to presidency, Lincoln vowed to champion for the emancipation of all slaves in the spirit of protecting human dignity under the ‘all men are equal’ mantra. The subsequent election of Abraham Lincoln angered a section of Southern states for fear that Lincoln would make good on his abolition pledge. In 1861, South Carolina alongside ten other Southern states wrote to the federal government declaring their intention to secede from the continental USA. Subsequently, the eleven states declared their secession after forming the Confederate States of America. The period that ensued is considered the mother of all internal conflicts. The resultant series of upheavals claimed the lives of thousands of American civilians and soldiers and leaving property worth millions of dollars destroyed. Slavery was central in the American civil war since the South remained adamant to relinquish their slaves even after Lincoln issued the historic Emancipation Proclamation after provoking his presidential powers. The president pushed for the inclusion of emancipation in the constitutional definition of liberty2. This marked the Thirteenth Amendment to the US Constitution. A frican American slaves fled from their owners and sought refuge in the Union camps as the war raged on. It is prudent to underscore the significance of the issue of slavery since it was the sole reason behind the conflict. April 1865 Going by the memoirs, books, journals and diaries of veteran soldiers, the period beginning April 9 to April 30 was poignantly etched in the minds of the entire nation. This was a period of uncertainty and the atmosphere was tense. Historians believe that

Decision making to IT related Ethical issue Essay

Decision making to IT related Ethical issue - Essay Example The latter consists of software programs, music, videos and other content that is being protected by the international law. The sites which provide easy access to it are called trackers. One of the most famous ones, namely The Pirate Bay has been closed several times, but it still operates. One would make no mistake that there are several groups of stakeholders that can be identified in this situation. First of all, there are end users who got used to being able to download any content they want for free. Secondly, there are the creators of the contents who want to earn money with the help of it. Thirdly, there is the government who is willing to gain an effective control over this segment. Finally, there are owners of the web sites that are affected by the applicable legislation. There may be several consequences of the decision. For example, if software piracy is being taken care of severely, it will be impossible to listen to the music online (Morley & Parker, 2007, p. 604). In addition to that, a lot of web site will have to erase the content that they currently have and change to comply with the law. There are several principles that should be taken into consideration. On the one hand, the government is responsible for making sure that its citizens obey the law and punish those who refuse to do so (Kehal, 2004, p. 176). In spite of the fact that the Internet is considered to be a virtual space that does not obey any particular government, those who download the content are always situated in a particular legal framework. On the other hand, there are people who argue that free sharing should become the characteristic feature of the digital age and it is ridiculous to fight it. One might identify quite a few options that there are. To begin with, it may be rather effective to block every single web site that provides those who access it with the ability to

Death Penalty annotated bibliography Example | Topics and Well Written Essays - 750 words

Death Penalty - Annotated Bibliography Example statistical information on how these individuals were brought to justice through death penalty, and focuses on information related to the race, sex and colour of the individuals who faced this sentence. It even provides statistical information of the states in which this penalty was levied and the offenses for which this penalty was levied. Cothern, L., United States., United States., & Coordinating Council on Juvenile Justice and Delinquency Prevention (U.S.). (2000). Juveniles and the death penalty. Washington, DC: U.S. Dept. of Justice, Office of Justice Programs, Office of Juvenile Justice and Delinquency Prevention. The subject of sentencing underage or juvenile individuals to death sentence or death penalty has been under discussion for a very long period of time. All most all the jurisdictions of USA, assign death sentence to individuals who are below 18 but the question of whether this sentence is consistent with the juvenile justice system of US has been asked. The report not only focuses on Death Penalty, it even pays attention to the history of death sentence for individuals of all ages and especially for juvenile. It even focuses on the movements that are opposing its existence and whether juvenile death sentence is justified or not. This resource provides information about death penalty incidents that have taken place in the jurisdiction of Kentucky State. This report focuses on the total cost involved in death penalty sentences, these costs does not only include social and emotional costs, these costs even include the financial burden that the state has to bare in cases of death penalty. This resource even provides information about the costs experienced by different states of US including Florida, Tennessee, California and Maryland and all the remaining states that are using this way of providing justice. This resource even provides statistical report on the number of people who have been sentenced for death penalty. Connecticut Repeals Death

The Science of Toxicology

The Science of Toxicology Introduction to Toxicology: The science of Toxicology consists of the study of biology, chemistry, and medicine, that is concerned with study of harmful; effects of chemicals on living organisms. It also studies the harmful effects of the chemical, biological and the physical agents in biological systems that establish the extent of damage in living organisms. The relationship between the given dose and its effects on the exposed organism is of very high significance in toxicology. Variables that influence chemical toxicity, includes the given dosage, the probable route of exposure, species, age, sex and environment. A toxicologist is a scientist or medical personal who specializes in the study and observation of symptoms, function and mechanism, treatments and detection of venoms and toxins; especially in case of poisoning. To work as toxicologist one should get a degree in toxicology or a related field like biochemistry and the life sciences. The main branches of toxicology are: Forensic toxicology: It is the use of toxicology and other disciplines such as pharmacology, chemistry such as analytical chemistry and clinical chemistry to aid medical or legal investigation of death due to poisoning, and drug use. The chief concern for forensic toxicology is not always the legal outcome of the toxicological investigation or the technology used, but rather the obtaining and interpreting of the evidence and results. A toxicological analysis now can be done to various kinds of samples. A forensic toxicologist must minutely consider the context of an investigation, particularly any physical symptoms that are recorded, and any evidences collected at scene of the crime that helps in narrowing the search, such as any available chemicals powders and/or trace residue. Armed with this information and samples with which to work, the toxic substances that are present there, its concentrations, the probable chemicals effects on the person, all of these information are determined by the forensic toxicologist. In vitro toxicity: It is the scientific analysis of the effects of toxic chemical substances on cell cultured bacteria or mammalian cells. These methods are used primarily to identify dangerous chemicals, to verify the lack of certain toxic properties in the early stages of development of potentially useful new substances like therapeutic drugs, agro chemicals, food colours and additives and other useful substances. In vitro assays for xenobiotic toxicity are carefully considered by major government organizatios (e.g. EPA, NTP, FDA), to better assess human risks. There are major activities in using in vitro systems to advance understanding of toxicant activities, and the use of human cells, tissues and organs to define human-specific toxic effects. Environmental toxicology: It is a multidisciplinary field of science concerned with study of the harmful effects of various chemical agents, biological agents and physical agents on living organisms. it is a sub discipline of environmental toxicology that is concerned with studying the harmful effects of toxicants, at the general population and ecosystem levels. Medical toxicology: It is a medical subfield focusing on the diagnosis of health problems, their management and prevention of adverse health effects such as poisoning and other complications from medications, occupational toxicants, toxicants in the environment, and/or various other biological agents. Medical toxicologists personal are involved in the assessment and treatment for poisoning, the harmful drug reaction, overdoses and substance abuse. Medical toxicology practitioners are physicians, whose primary specialization is generally in emergency medicine, occupational medicine or pediatrics. Ecotoxicology: It is the study of the effects of toxic chemicals on the biological organisms, at the population, community and at the ecosystem levels. Study of Ecotoxicology is a multidisciplinary field, which combines toxicology and ecology. The ultimate aim of this approach is to be able to predict the effects of pollution so that efficient and effective action to prevent or remediate any adverse effect can be identified. In the ecosystems that are already affected by pollution, Eco toxicological studies can inform as to the best method for action to restore the ecosystem efficiently and effectively. Ecotoxicology differs from science of environmental toxicology in that it combines the effects of stressors across all the levels of biological organizations i.e. from the molecular to whole communities and ecosystems, whereas science of environmental toxicology focuses upon the effects at level of the individual and below. Entomotoxicology: It is the analysis of toxins in arthropods that feed on carrion. Using arthropods in corpse or at crime scene, investigators can correctly determine whether toxins or poisons were present in a body at the exact time of death. This technique is a major advancement in forensics. Before, such determinations were impossible in the case of the severely decomposed bodies, which were devoid of intoxicated tissue and body fluids. Ongoing researches into the effects of toxins on arthropod and their development has also allowed better estimations of the postmortem intervals. Forensic entomology is the application and also the study of insects and other arthropod biology to criminal matters. It also involves application of study of arthropods, such as insects, the arachnids, the centipedes, and millipedes, crustaceans to the criminal or legal proceedings. It is mainly associated with death investigations; however, it may also be used to detect drugs, poisons and determine the location of an incident, and also find the presence and time of when the wounds were caused. Forensic entomology can thus be further broken under three subparts: urban, stored-product and lastly medico-legal/medico-criminal entomology. Toxinology: It is the specialized field of science that deals mainly with the animals, plants, and microbial toxins. It has been defined as the scientific discipline dealing with microbial toxins, plant toxins, and animal venoms. This involves more than just the chemistry and mode of action of toxins. It deals with the working of venom, the poison-producing organisms, also the structure and functions of the venom glands, use of the venom or poison and also the ecological role of these compounds. Toxinology has also been further defined as the science of toxic substances produced by or stored in living organisms, their properties, and their biological importance for the organisms involved. Clinical toxinology: Within toxinology there is also a subgroup, i.e. clinical toxinologists, who studies the medical effects in humans, exposure to the toxins, also in animal venoms or in plant poisons. This includes problems such as venom from snakebite, currently considered to affect more than 2.5 million patients each year, with over more than 100,000 deaths. Clinical toxinology does not have specialist status yet within the field of medicinal study, unlike other fields such as surgery and radiology. However, training courses in clinical toxinology exists. Sample Preparation: Sample preparation is often the first step in an analysis; the result of this step can affect the rest of the analytical process. To get accurate results, a sample should be representative, it should be reproducible, homogenous, and must be suitable for column injection or other assay. The main steps in sample preparation are: Sample Identification Sample reagent and standard pipetting Sample extraction Output to analyzer format Preparative Steps: Removal of Soluble Protein – precipitation – filtration Extraction – single step liquid-liquid extraction – Multiple step liquid-liquid extraction (â€Å"back-extraction†) – solid phase extraction Chemical Modification – derivatization for increase in volatility of sample – chemical hydrolysis of glucuronide enzyme Concentration – evaporation Cell lysis or tissue homogenation Sample Characterization: There are many chromatographic assays (GC, GC/MS, HPLC, TLC, LC/MS/MS, ), that are used for characterization and toxicological analysis of sample. To understand them, it is best to break them down into their modular components/steps: Sample preparation Separation (the actual chromatography) Detection (UV/Vis spectrometry, Fluorescence spectrometry, Mass spectrometry). Chromatographic Components: Sample â€Å"loading† The â€Å"mobile phase† during separation. The â€Å"stationary phase† during separation. Separation of individual molecules in the sample components is always based on their relative affinity for the mobile phase versus the stationary phases. Because some of the molecules have higher affinity for the stationary phase, they will pass through column slower than the others and, therefore, will be separated. Separation of the different Molecules by Chromatography: After the injection, all molecules start out overlapping. Due to the varying relative affinity for the stationary phase versus the mobile phases, individual molecules thus begin to separate As the different molecules then elute off of the column, they are then detected as resolved â€Å"peaks†. Relative Retention Times: During the separation, the absolute rates/times for movement of the molecules are not always reproducible. For example, the columns can get dirty, thus decreasing the amount of stationary phase that is available for the interaction with molecules. This can be compared to shortening the length of the column. However, it affect the rate and all molecules in the same way. Therefore, their relative rates/times are highly reproducible. The â€Å"relative retention time† (RRT) is defined as the detection time for a individual peak divided by the detection time for a known internal standard. RRTs are characteristic and reproducible identifiers of individual molecules. Quantification of Drug Concentrations: Peak â€Å"area† generally correlates with the amount of drug that is loaded onto a column and on the original drug concentration. But, there can be sample-to-sample variations due to the extraction efficiency, the loading volumes, or the detection efficiency, etc. Again, the internal standard is utilized to correct for variations.–Similar to the relative retention time, relative peak intensity is defined and related to drug concentration. Unlike the relative retention time, the given variation in the peak area is not always similar for all the molecules. Thus, the internal standard is chosen to be chemically similar to the analyte of interest to best correct for variations. However, adequate similarity is not easy to predict or establish. Protocol for Quantification of Analyte Concentration Based Upon a Calibration Curve: A known quantity of an internal standard is first added to every sample (including controls and calibrators) before any other preparative step. All samples are then prepared through the identical preparative steps, separated by a chromatographic method and quantitatively detected. The relative peak intensities are measured for a series of calibrators with a fixed amount of internal standard and varying amounts of a known analyte. These relative peak intensities are fit to an equation, generally linear, to define a calibration curve. The relative peak intensities of unknown samples are then calculated and then related to the calibration curve to quantify the concentration of the analyte(drug) in the original clinical sample. Some Characterization Techniques: Affinity Chromatography: Affinity chromatography is used for separating biochemical mixtures based on the highly specific interaction between conjugates such as that between antigens and antibodies, enzymes and substrates, or receptors and ligands. Principle: Here, the stationary phase used is typically a gel matrix, often of agarose. Generally, we use an undefined heterogeneous group of molecules in solution, like, for example, growth medium or blood serum. The molecule of interest will be having a well-defined property, and can be put to use during the affinity purification process. This process can thus be seen as a process of entrapment, with target molecule getting entrapped on solid or stationary phase and/ or medium. The molecules of mobile phase component will not become trapped as they do not possess this property. The stationary phase is then removed from the mixture, washed and target molecule released from entrapment in process known as elution. The most common use of affinity chromatography is for the purification of recombinant proteins. Affinity chromatography has use in number of applications, including purification from nucleic acid, and purification from blood and also protein purification from cell free extracts. Thin-layer chromatography (TLC): It is a chromatography technique used to separate non-volatile and stable mixtures. Thin-layer chromatography analysis is performed on sheet of various mediums, such as glass, plastic, or aluminum foil, they are then coated with a thin layer of adsorbent material, like silica gel, cellulose and also aluminum oxide. This layer is known as the stationary phase. After the sample is applied on the plate, a solvent or solvent mixture (known as the mobile phase) is drawn up the plate via capillary action. Because different analytes have different rate of ascension on the TLC plate, separation is achieved. It can monitor the progress of a reaction, or determine the purity of substances and/or identify the compounds present in a given mixture. Some examples are: analyzing the fatty acids, detection of pesticides ,herbicides and/or insecticides in food and water, analyzing ceramides, analyzing the dye composition of fibers in forensic toxicology, or identification of medicinal plants and their constituents and assaying the radiochemical purity of radiopharmaceuticals. A number of enhancements to the original method have been made, to increase the resolution achieved with TLC, to make the different steps automatic and to allow more accurate quantitative analysis. This is called HPTLC, or high-performance TLC. Summary of Major Learning Points: Modular nature of chromatograpy. – Assays are divided into three steps: sample preparation, sample component separation and analyte detection. – The separation steps consist of sample loading, preparing a mobile phase and a stationary phase. Importance of an internal standard for – Calculating the relative retention times for component separation. – Calculation of the relative peak areas and the generation of a calibration curve for the quantification of drug concentrations in the original clinical sample. Analytical specificity provided by – Sample preparation techniques – Separation during chromatography (RRT) – Method chosen for detection

Sunrise On The Veldt: Order :: essays research papers

Sunrise on the Veldt: Order Order is sought instinctively. In Literature, as well as Biology, order is sought instinctively by authors and scientists. Authors use order to convey real-life incidents and make their stories seem more realistic. Scientists use a way of classification to bring order to Biology. The life cycle, as the cycle of a virus, shows order. The young boy in the short story, "Sunrise on the Veldt," found order in the life cycle. He sought this order to help him explain the death of a buck. The death of the buck made the young boy think about the life cycle. He shot the buck, and the buck became injured. Then the buck died. An organism is born, it grows, it lives for a period of time, then it dies. The human life cycle is similar. A baby is born. The baby’s parents take care of it, then the baby turns into an adult. The adult lives for a period of time, then the adult dies. Humans seek order in the life cycle to help explain death. The order in the life cycle was sought instinctively, because people wanted an explanation of death. The order in "Sunrise on the Veldt" was shown in the life cycle. In the novel, The Wave, a teacher sought order to help keep his classroom under control. The order helped keep the classroom under control. But the students began to notice they were not thinking, and the order began to tear the school apart. The teacher sought order because he wanted his students to behave better. Scientists use order to control viruses. Viruses are classified by several attributes; their shape, the vectors that transmit them, and their RNA or DNA content. Once a virus is classified, it can be examined, and controlled. Biologists use order to classify other organisms as well. Charles Darwin sought order instinctively by becoming a naturalist. He studied animals and plants and devised a theory of evolution. He decided that variations exist within populations. Some variations are more advantageous for survival and reproduction than others. Organisms produce more offspring than can survive. Over time, offspring of survivors will make up a larger proportion of the population. Darwin believed that organisms produce more offspring so that the stronger offspring can live, but the weak offspring die. Darwin, the virus cycle, and The Wave portray order being sought instinctively. A virus seeks order instinctively. A virus attaches itself to a host. Then it enters into the host by exchanging its DNA or RNA. The virus then replicates itself, inside the host.

Normal distribution

Therefore, she wanted to know more about her course mates. Obviously it was difficult to contact all the students from her batch and know about them. So she decides to collect a randomly selected data from about 25-30 students. She was not sure about random collection of data. So she learnt about random number generator and used student roll numbers to select the random sample. With the help of faculty she prepared questionnaire and collected data. From the data she prepared report for submission. It was quite nice and elaborate. However, when she compared it with friends, she found lot of variation. So she decided to take information from her two friends who had also collected the similar data.Of course she has to take some precautions while using the data from the friends. A) b) c) d) e) f) g) h) Collect the data. Draw Simple Bar Diagram to represent specialization wise strength of students. Draw Pie diagram to represent the number of students in each of the blood groups. Prepare t he gender-wise frequency table for the blood groups. Plot graph of height Vs.. Weight of the students. Comment on the graph. Plot graph of family income Vs.. Expenditure of the student. Comment on the graph. Calculate gender-wise mean and standard deviation of marks of 10th and 12th standard. Comment on the basis of the results. Prepare a two way table showing age-wise and gender-wise strength of students.Represent this data with the help of a multiple bar diagram taking age on X axis and gender-wise strength on Y axis. Also comment on the basis of this graph. Prepare a two way table showing father's education and mother's education taking education categories as Below Graduate, Graduate and Post graduate. Comment on the basis of this table. Calculate mean and standard deviation of marks obtained by the students in 10th and 12th standard according to having a Job experience or not. Comment on the values you obtained. You are free to do additional analysis if you feel so. K) Note: Sa mple questionnaire is attached. However, please feel free to add few question based on your purpose.Subject: Statistics & Research Methodology (103) Case No: 04 Microchip Contract A company receives an order for five custom-made microchips at a price of $7,500 each. The company will produce the chips one by one using a complex process which has only a 67% chance of producing a defect-free chip at each trial. After five defect- free chips are produced the process will be stopped. A cost accountant at the company has prepared the following cost report: The cost of production includes a $14,800 fixed cost and a $2,XX unit variable cost. Thus if X number of chips are produced, the total cost of production would be 14,800 + 2. XX dollars. The revenue minus the cost of production will be the profit.After some analysis the finance manager of the company says that the risk may be too high and thinks the order should not be accepted. 1. What distribution will the number of chips produced, X, follow? 2. What is the expected value and standard deviation of X? 3. What is the expected value and standard deviation of the profit? 4 What is the break-even X (allow fractional values for X)? 5. What is the probability that accepting the order will result in a loss? 6. A popular measure of risk in a venture is value at risk, which is the joss suffered at the 5th percentile of the return from the venture. In this problem, find an integer x such that P[X>x] is approximately 5%. 7. For the x value found in part 6, calculate the loss, and thus the value at risk. . Express the value at risk as a percentage of the expected value of the profit. 9. What is your assessment of the risk and reward in the order? Should the company accept the order? The sales manager of the company says that the customer is very likely to agree to increase the order quantity from five to eight chips. But he is not sure whether the matter should be pursued with the customer. 10. If accepting an order of five itself is risky, will it not be even more risky to accept an order for eight? † asks the sales manager. How would 1 1 . Calculate the expected value and standard deviation of the profit for an order quantity of eight. 12.What is the value at risk for an order quantity of eight, computed in a manner similar to parts 6 and 7 above? Express the value at risk as a percentage of the expected profit. 13. Looking at the answer to parts 3,8,1 1, and 12, would you say the risk and reward have become more favorable, compared to an order quantity of five? 14. Should the company pursue the matter of increasing the order quantity to eight with the customer? * Case taken from Complete Business Statistics by Emir Cell &J. Countermanding, DATA Mac Grab Hill. Subject: Statistics & Research Methodology (103) Case No: 05 Acceptable Pins A company supplies pins in bulk to a customer. The company uses an automatic lathe to produce the pins.Due to many causes- vibrations, temperature, wear and tear , and the like-the lengths of the pins made by the machine are normally distributed with a mean of 1. 012 inches and a standard deviation of 0. 018 inch. The customer will buy only those pins with lengths in the interval 1. 00 Ð’Â ± 0. 2 inch. In other words, the customer wants the length to be 1. 00 inch but will accept up to 0. 02 inch deviation on either side. This 0. 02 inch is known as the tolerance. 1 . What percentage of the pins will be acceptable to the consumer? In order to improve percentage accepted, the production manager and the engineers discuss adjusting the population mean and standard deviation of the length of the pins. 2.If the lathe can be adjusted to have the mean of the lengths to any desired value, what should it be adjusted to? Why? 3. Suppose the mean cannot be adjusted, but the standard deviation can be reduced. What maximum value of the standard deviation would make 90% of the parts acceptable to the consumer? (Assume the mean to be 1. 012. ) 4. Repea t question 3, with 95% and 99% of the pins acceptable. 5. In practice, which one do you think is easier to adjust, the mean or the standard deviation? Why? The production manager then considers the costs involved. The cost of resetting the machine to adjust the population mean involves the engineers' time and the cost of production time lost.The cost of reducing the population standard deviation involves, in addition to these costs, the cost of overhauling the machine and engineering the process. 6. Assume it costs $xx to decrease the standard deviation by (x 11000) inch. Find the cost of reducing the standard deviation to the values found in question 3 and 4. 7. Now assume that the mean has been adjusted to the best value found in question 2 at a cost of $80. Calculate the reduction in standard deviation necessary to have 90%, 95%, and 99% of the parts acceptable. Calculate the respective costs, as in questions. 8. Based on your answers to questions 6 and 7, what are your recommend ed mean and standard deviation? Normal distribution Therefore, she wanted to know more about her course mates. Obviously it was difficult to contact all the students from her batch and know about them. So she decides to collect a randomly selected data from about 25-30 students. She was not sure about random collection of data. So she learnt about random number generator and used student roll numbers to select the random sample. With the help of faculty she prepared questionnaire and collected data. From the data she prepared report for submission. It was quite nice and elaborate. However, when she compared it with friends, she found lot of variation. So she decided to take information from her two friends who had also collected the similar data.Of course she has to take some precautions while using the data from the friends. A) b) c) d) e) f) g) h) Collect the data. Draw Simple Bar Diagram to represent specialization wise strength of students. Draw Pie diagram to represent the number of students in each of the blood groups. Prepare t he gender-wise frequency table for the blood groups. Plot graph of height Vs.. Weight of the students. Comment on the graph. Plot graph of family income Vs.. Expenditure of the student. Comment on the graph. Calculate gender-wise mean and standard deviation of marks of 10th and 12th standard. Comment on the basis of the results. Prepare a two way table showing age-wise and gender-wise strength of students.Represent this data with the help of a multiple bar diagram taking age on X axis and gender-wise strength on Y axis. Also comment on the basis of this graph. Prepare a two way table showing father's education and mother's education taking education categories as Below Graduate, Graduate and Post graduate. Comment on the basis of this table. Calculate mean and standard deviation of marks obtained by the students in 10th and 12th standard according to having a Job experience or not. Comment on the values you obtained. You are free to do additional analysis if you feel so. K) Note: Sa mple questionnaire is attached. However, please feel free to add few question based on your purpose.Subject: Statistics & Research Methodology (103) Case No: 04 Microchip Contract A company receives an order for five custom-made microchips at a price of $7,500 each. The company will produce the chips one by one using a complex process which has only a 67% chance of producing a defect-free chip at each trial. After five defect- free chips are produced the process will be stopped. A cost accountant at the company has prepared the following cost report: The cost of production includes a $14,800 fixed cost and a $2,XX unit variable cost. Thus if X number of chips are produced, the total cost of production would be 14,800 + 2. XX dollars. The revenue minus the cost of production will be the profit.After some analysis the finance manager of the company says that the risk may be too high and thinks the order should not be accepted. 1. What distribution will the number of chips produced, X, follow? 2. What is the expected value and standard deviation of X? 3. What is the expected value and standard deviation of the profit? 4 What is the break-even X (allow fractional values for X)? 5. What is the probability that accepting the order will result in a loss? 6. A popular measure of risk in a venture is value at risk, which is the joss suffered at the 5th percentile of the return from the venture. In this problem, find an integer x such that P[X>x] is approximately 5%. 7. For the x value found in part 6, calculate the loss, and thus the value at risk. . Express the value at risk as a percentage of the expected value of the profit. 9. What is your assessment of the risk and reward in the order? Should the company accept the order? The sales manager of the company says that the customer is very likely to agree to increase the order quantity from five to eight chips. But he is not sure whether the matter should be pursued with the customer. 10. If accepting an order of five itself is risky, will it not be even more risky to accept an order for eight? † asks the sales manager. How would 1 1 . Calculate the expected value and standard deviation of the profit for an order quantity of eight. 12.What is the value at risk for an order quantity of eight, computed in a manner similar to parts 6 and 7 above? Express the value at risk as a percentage of the expected profit. 13. Looking at the answer to parts 3,8,1 1, and 12, would you say the risk and reward have become more favorable, compared to an order quantity of five? 14. Should the company pursue the matter of increasing the order quantity to eight with the customer? * Case taken from Complete Business Statistics by Emir Cell &J. Countermanding, DATA Mac Grab Hill. Subject: Statistics & Research Methodology (103) Case No: 05 Acceptable Pins A company supplies pins in bulk to a customer. The company uses an automatic lathe to produce the pins.Due to many causes- vibrations, temperature, wear and tear , and the like-the lengths of the pins made by the machine are normally distributed with a mean of 1. 012 inches and a standard deviation of 0. 018 inch. The customer will buy only those pins with lengths in the interval 1. 00 Ð’Â ± 0. 2 inch. In other words, the customer wants the length to be 1. 00 inch but will accept up to 0. 02 inch deviation on either side. This 0. 02 inch is known as the tolerance. 1 . What percentage of the pins will be acceptable to the consumer? In order to improve percentage accepted, the production manager and the engineers discuss adjusting the population mean and standard deviation of the length of the pins. 2.If the lathe can be adjusted to have the mean of the lengths to any desired value, what should it be adjusted to? Why? 3. Suppose the mean cannot be adjusted, but the standard deviation can be reduced. What maximum value of the standard deviation would make 90% of the parts acceptable to the consumer? (Assume the mean to be 1. 012. ) 4. Repea t question 3, with 95% and 99% of the pins acceptable. 5. In practice, which one do you think is easier to adjust, the mean or the standard deviation? Why? The production manager then considers the costs involved. The cost of resetting the machine to adjust the population mean involves the engineers' time and the cost of production time lost.The cost of reducing the population standard deviation involves, in addition to these costs, the cost of overhauling the machine and engineering the process. 6. Assume it costs $xx to decrease the standard deviation by (x 11000) inch. Find the cost of reducing the standard deviation to the values found in question 3 and 4. 7. Now assume that the mean has been adjusted to the best value found in question 2 at a cost of $80. Calculate the reduction in standard deviation necessary to have 90%, 95%, and 99% of the parts acceptable. Calculate the respective costs, as in questions. 8. Based on your answers to questions 6 and 7, what are your recommend ed mean and standard deviation?

Overcoming Writer’s Block by Bryan Hutchinson

Overcoming Writer’s Block by Bryan Hutchinson Overcoming Writer’s Block: An Interview with Bryan Hutchinson We often like to say that writing the book is the hard part. Sometimes, though, it can get really hard. Writer’s block is an ever-present subject in writing forums and communities, and most writers are faced with it at some point in their careers.There are no simple tricks and techniques for overcoming writer’s block that work for everyone, contrarily to what the myriad of blog posts on this subject would lead you to believe. It is, however, almost always linked to self-doubt (or to an underlying block), and that is something everyone (not just writers) can work on.For this, we decided to cut through all the noise out there around this subject and bring a true expert to the Reedsy blog: Bryan Hutchinson is the author of Writer’s Doubt and the founder of Positive Writer, one of the most acclaimed blogs about writing out there. What’s good about him is that he is, indeed, positive. And self-doubt has little grip on a positive person. Self-doubt affects everyone in every aspect of their lives. I’ve heard from people in a lot of different fields who have read my book â€Å"Writer’s Doubt† and found it extremely helpful and they’re surprised because it is supposed to be for writers. The reality is we all deal with doubt and I just happened to write about it in the context of writing, but anyone reading the book can apply it to the context of their lives and their work.Follow Bryan Hutchinson and Reedsy on Twitter: @Adderworld and @ReedsyHQHave you ever been faced with writer's block? How do you usually deal with it (over here at Reedsy, we just go for a walk)? Leave us your experiences and tips, or any question for Bryan, in the comments below!

Free Essays on Themes From Lord Of The Flies

There are several themes in Lord of the Flies that can be related to everyday government. Everyone is fighting. There is a particular group that opposes other’s beliefs. This group feels that their way is the best way to get things accomplished. Some things that are brought to attention in this film are unity, democracy, liberty and freedom, and individualism. The boys in the film thought that they had to work together as one in order to get off the island. Others did not care if they got off the island and back to their families. First, the group tried working together by gathering food, building shelters to sleep under, and keeping a fire aflame at all times so that maybe they would be spotted by someone flying over or passing by. Some form of government had to be established to keep everyone unified. The group decided they needed a leader. Some thought that Jack should be the leader since he was the oldest in the group. Others thought that Ralph should be the leader since he was already the colonel. Ralph was elected leader since he was the leader at the military school where they were all enrolled. Jack believed that everyone should have the liberty to do what he or she wanted without being reprimanded for it. He decided to be a rebel against what Ralph stood and what he was trying to accomplish. Jack started up what he called â€Å"his gang† and he had several people following him. This is very much like today’s government. There is a leader and followers. Others got tired of Ralph and all of his rules and decided to join Jack’s gang. Piggy was the only one who remained loyal to Ralph and all of his ideas. They shared the same morals and beliefs. They all had their own ideas on how things should be done. Some were too shy to voice their opinions. Jack voiced his feeling and followed up on it by doing whatever he wanted. Ralph let his opinion be known as well. The others just took sides with w... Free Essays on Themes From Lord Of The Flies Free Essays on Themes From Lord Of The Flies There are several themes in Lord of the Flies that can be related to everyday government. Everyone is fighting. There is a particular group that opposes other’s beliefs. This group feels that their way is the best way to get things accomplished. Some things that are brought to attention in this film are unity, democracy, liberty and freedom, and individualism. The boys in the film thought that they had to work together as one in order to get off the island. Others did not care if they got off the island and back to their families. First, the group tried working together by gathering food, building shelters to sleep under, and keeping a fire aflame at all times so that maybe they would be spotted by someone flying over or passing by. Some form of government had to be established to keep everyone unified. The group decided they needed a leader. Some thought that Jack should be the leader since he was the oldest in the group. Others thought that Ralph should be the leader since he was already the colonel. Ralph was elected leader since he was the leader at the military school where they were all enrolled. Jack believed that everyone should have the liberty to do what he or she wanted without being reprimanded for it. He decided to be a rebel against what Ralph stood and what he was trying to accomplish. Jack started up what he called â€Å"his gang† and he had several people following him. This is very much like today’s government. There is a leader and followers. Others got tired of Ralph and all of his rules and decided to join Jack’s gang. Piggy was the only one who remained loyal to Ralph and all of his ideas. They shared the same morals and beliefs. They all had their own ideas on how things should be done. Some were too shy to voice their opinions. Jack voiced his feeling and followed up on it by doing whatever he wanted. Ralph let his opinion be known as well. The others just took sides with w...

The Epistle to Philippians Essay Example | Topics and Well Written Essays - 750 words

The Epistle to Philippians - Essay Example First of all, it would be advantageous to see if there are lots of similarities between the above mentioned versions. One might argue that there are many parts of the text that are similar word for word: the very beginning, some parts in the end and many others. However, its interesting to note that there are two words, namely â€Å"true† and â€Å"pure†, that coincide in the list of what a Christian should think about, while others are not similar at all. Indeed, there are more differences in the two texts than similarities. For example, NIV says that Paul â€Å"pleads† two women to keep their faith strong, while NRSV uses the word â€Å"urges†. It is clear that the latter verb has far stronger meaning. Another difference is seen in the words that are used to modify the word â€Å"companion†: NIV’s â€Å"true† versus NRSV’s â€Å"loyal†. One would make no mistake arguing that the former word has some important connotatio ns. The next suitable example is the difference between the phrases â€Å"do not be anxious† (NIV) and â€Å"do not worry† (NRSV). From a psychological point of these two states of mind are different. Now it would be important to perform some exegetical analysis. In the very beginning, Paul greets the congregation warmly. Then he encourages them to stand firmly in their faith in Jesus Christ. Afterwards, he calls two names of the women who need extra support since they might be falling from grace; that is why the believers should unite together. The next statement urges the Christians to rejoice in God all the time. The Apostle says that God is near. That is why the believers should not worry about anything and pray to God all the time, presenting Him with their concerns. In this case the peace of God will come to them. In the end, Paul lists what Christians should think about: these are universally accepted positive values. Finally, he urges all the believers to put into practice their knowledge and enjoy their faith.  The passage from this book is related to the New Testament in that sense that it reflects the spreading of Christian Church and tries to reinforce the newly fou nd faith. It is also related to the Old Testament in that aspect that people should base their life firmly on their faith in God.

Statement of Interest Essay Example | Topics and Well Written Essays - 250 words

Statement of Interest - Essay Example That marked a day that not only changed the United States as a nation but also inevitably awakened in me a deeper consciousness of the necessity in addressing this ubiquitous issue. I intend to use the knowledge that I have gained in understanding terrorism in correlation to international relations to more substantial application that goes beyond the four corners of the classroom. As a nation, it had been an imperative function for us to communicate and develop ties with other nations. In the process, there had been friendships that were forged and there had been those, countries or groups, who reviled such extension of amity. Security is an integral fact that must be given due attention in our diplomatic discourse. To this end, I know that there is no better opportunity for me than to work and train under your good office. I have been working as an emergency medical technician in the past six years and in that period of time I have learned that there is nothing more precious than human life. The inhumane acts of terrorism serve no purpose but to instil fear in innocent civilians. This is where my fervour toward counterterrorism and the indispensability of security